Enzymes and Inhibitors
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, 100 MG, Purified precrystalline DNase. A lyophilized powder. Store at 2-8degreeC., Source: Bovine Pancreas, Minimum Activity: =2,000 Kunitz units per mg dry weight
Vector Laboratories ImmPACT DAB Peroxidase (HRP) Substrate Kit 120 ml
Vector Laboratories ImmPACT DAB HRP Substrate Kit produces a dark brown reaction product that is crisper and 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4 degrees C. This product consists of a concentrated stock solution of highly purified DAB and 120 ml of diluent and can be used for IHC and on blots. Vector Laboratories horseradish peroxidase (HRP) substrate kits include options that are cost-effective (e.g. standard DAB), sensitive (e.g. ImmPACT DAB) and convenient (ImmPACT DAB EqV). The range of vibrant colors available facilitates multiplex staining.
New England Biolabs, Inc. HindIII-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsmBI-v2 – 200 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN and is optimized for Golden Gate Assembly.
- Time-Saver qualified for digestion in 5-15 minutes
- Used in Golden Gate Assembly
- Type IIS Res recognize asymmetric DNA sequences; cleave outside of their recognition site
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. BbsI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2^. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Surmodics Inc TMB One Component HRP Microwell Substrate, 1L
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
New England Biolabs, Inc. KpnI-HF – 4000 units
A high fidelity restriction endonuclease that recognizes the sequence G_GTAC^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. MluI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Sigma Aldrich Fine Chemicals Biosciences DNASE I GRADE II, FROM BOVINE
DNase I grade II, from bovine pancreas 100mg
New England Biolabs, Inc. AgeI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence A^CCGG_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Sigma Aldrich Fine Chemicals Biosciences DNase I, 100 mg
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Accelagen TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
New England Biolabs, Inc. Rapid™ PNGase F – 50 reactions
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
Worthington Biochemical Corporation PAPAIN DISSOCIATION SYSTEM
Papain Dissociation System, 100mL of EBBS, 2degree to 8degreeC Storage Temp, 1BX
LGC GENOMICS LLC (Lucigen) Proteinase K, 2 mLDigest proteins during DNA and RNA purification or inactivate enzymes. To be used with the MasterPure Kits.
This reagent is designed for use with the MasterPure™ kit protocols only.